{"ModuleCode":"CN4247R","ModuleTitle":"Enzyme Technology","Department":"Chemical & Biomolecular Engineering","ModuleDescription":"This module will start with general introduction about enzyme, enzymatic transformation, and enzymatic process. It will be followed by various components in the development of an enzymatic process: enzyme classes and enzymatic reactions; enzyme discovery and high-throughput screening and detection methods; enzyme purification, characterisation, structure, function, and selectivity; protein engineering; cell engineering; biotransformation with isolated enzymes and microbial cells; reaction engineering; enzyme in organic solvent, two-liquid phase system, and enzyme stabilisation; cofactor regeneration; and product recovery. 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enzyme technologies for industrial production of chemicals via biotransformation. This module will start with general introduction about enzyme, enzymatic transformation, and enzymatic process. It will be followed by various components in the development of an enzymatic process: enzyme classes and enzymatic reactions; enzyme discovery and high-throughput screening and detection methods; enzyme purification, characterization, structure, function, and selectivity; protein engineering; cell engineering; biotransformation with isolated enzymes and microbial cells; reaction engineering; enzyme in organic solvent, two-liquid phase system, and enzyme stabilization; cofactor regeneration; and product recovery. Finally, the students will learn process economics and industrial examples on the enzymatic production of fine chemicals.
\r\n
\r\nModule Learning Outcomes:
\r\nOn successful completion of this module, students will be able to:
\r\n
\r\n\t- \r\n\t\tExplain different types of enzyme-catalysed transformations;
\r\n\t- \r\n\t\tDevelop suitable methods for enzyme discovery, engineering and purification;
\r\n\t- \r\n\t\tAnalyze enzyme activity, kinetics, and enantioselectivity, and evaluate cost of bioproduct manufacture;
\r\n\t- \r\n\t\tDesign biotransformations with isolated enzyme or whole cells as catalysts;
\r\n\t- \r\n\t\tInvestigate problems on biocatalytic process development.
\r\n\t\t \r\n
\r\n","Order":1},{"ID":"2e5f053b-8835-4692-be49-41f07234cfff","Title":"Prerequisites","Description":"CN2116, LSM1401","Order":2},{"ID":"3e5f053b-8835-4692-be49-41f07234cfff","Title":"Teaching Modes","Description":" This module consists of 3 h Lecture and 1 h Tutorial per week.","Order":3},{"ID":"4e5f053b-8835-4692-be49-41f07234cfff","Title":"Schedule","Description":"\r\n\t\r\n\t\t\r\n\t\t\t\r\n\t\t\t\t\r\n\t\t\t\t\t\r\n\t\t\t\t\t\t\r\n\t\t\t\t\t\t\t\r\n\t\t\t\t\t\t\t\tWeek | \r\n\t\t\t\t\t\t\t\r\n\t\t\t\t\t\t\t\tMon. (9:00-11:00) \r\n\t\t\t\t\t\t\t\tE5-03-21 | \r\n\t\t\t\t\t\t\t\r\n\t\t\t\t\t\t\t\tTue. (9:00-10:00) \r\n\t\t\t\t\t\t\t\tE5-03-21 | \r\n\t\t\t\t\t\t\t\r\n\t\t\t\t\t\t\t\tTue. (10:00-11:00) \r\n\t\t\t\t\t\t\t\tE5-03-21 | \r\n\t\t\t\t\t\t \r\n\t\t\t\t\t\t\r\n\t\t\t\t\t\t\t\r\n\t\t\t\t\t\t\t\tWeek 1 | \r\n\t\t\t\t\t\t\t\r\n\t\t\t\t\t\t\t\tLecture | \r\n\t\t\t\t\t\t\t\r\n\t\t\t\t\t\t\t\tLecture | \r\n\t\t\t\t\t\t\t\r\n\t\t\t\t\t\t\t\t- | \r\n\t\t\t\t\t\t \r\n\t\t\t\t\t\t\r\n\t\t\t\t\t\t\t\r\n\t\t\t\t\t\t\t\tWeek 2 | \r\n\t\t\t\t\t\t\t\r\n\t\t\t\t\t\t\t\tLecture | \r\n\t\t\t\t\t\t\t\r\n\t\t\t\t\t\t\t\tLecture | \r\n\t\t\t\t\t\t\t\r\n\t\t\t\t\t\t\t\tTutorial | \r\n\t\t\t\t\t\t \r\n\t\t\t\t\t\t\r\n\t\t\t\t\t\t\t\r\n\t\t\t\t\t\t\t\tWeek 3 | \r\n\t\t\t\t\t\t\t\r\n\t\t\t\t\t\t\t\tLecture | \r\n\t\t\t\t\t\t\t\r\n\t\t\t\t\t\t\t\tLecture | \r\n\t\t\t\t\t\t\t\r\n\t\t\t\t\t\t\t\tTutorial | \r\n\t\t\t\t\t\t \r\n\t\t\t\t\t\t\r\n\t\t\t\t\t\t\t\r\n\t\t\t\t\t\t\t\tWeek 4 | \r\n\t\t\t\t\t\t\t\r\n\t\t\t\t\t\t\t\tLecture | \r\n\t\t\t\t\t\t\t\r\n\t\t\t\t\t\t\t\tLecture | \r\n\t\t\t\t\t\t\t\r\n\t\t\t\t\t\t\t\tTutorial | \r\n\t\t\t\t\t\t \r\n\t\t\t\t\t\t\r\n\t\t\t\t\t\t\t\r\n\t\t\t\t\t\t\t\tWeek 5 | \r\n\t\t\t\t\t\t\t\r\n\t\t\t\t\t\t\t\tLecture | \r\n\t\t\t\t\t\t\t\r\n\t\t\t\t\t\t\t\tLecture | \r\n\t\t\t\t\t\t\t\r\n\t\t\t\t\t\t\t\tTutorial | \r\n\t\t\t\t\t\t \r\n\t\t\t\t\t\t\r\n\t\t\t\t\t\t\t\r\n\t\t\t\t\t\t\t\tWeek 6 | \r\n\t\t\t\t\t\t\t\r\n\t\t\t\t\t\t\t\tLecture | \r\n\t\t\t\t\t\t\t\r\n\t\t\t\t\t\t\t\tTest 1 | \r\n\t\t\t\t\t\t\t\r\n\t\t\t\t\t\t\t\tTutorial | \r\n\t\t\t\t\t\t \r\n\t\t\t\t\t\t\r\n\t\t\t\t\t\t\t\r\n\t\t\t\t\t\t\t\tRecess Week | \r\n\t\t\t\t\t\t\t\r\n\t\t\t\t\t\t\t\t- | \r\n\t\t\t\t\t\t\t\r\n\t\t\t\t\t\t\t\t- | \r\n\t\t\t\t\t\t\t\r\n\t\t\t\t\t\t\t\t- | \r\n\t\t\t\t\t\t \r\n\t\t\t\t\t\t\r\n\t\t\t\t\t\t\t\r\n\t\t\t\t\t\t\t\tWeek 7 | \r\n\t\t\t\t\t\t\t\r\n\t\t\t\t\t\t\t\tLecture | \r\n\t\t\t\t\t\t\t\r\n\t\t\t\t\t\t\t\tLecture | \r\n\t\t\t\t\t\t\t\r\n\t\t\t\t\t\t\t\tTutorial | \r\n\t\t\t\t\t\t \r\n\t\t\t\t\t\t\r\n\t\t\t\t\t\t\t\r\n\t\t\t\t\t\t\t\tWeek 8 | \r\n\t\t\t\t\t\t\t\r\n\t\t\t\t\t\t\t\teLearning | \r\n\t\t\t\t\t\t\t\r\n\t\t\t\t\t\t\t\teLearning | \r\n\t\t\t\t\t\t\t\r\n\t\t\t\t\t\t\t\teLearning | \r\n\t\t\t\t\t\t \r\n\t\t\t\t\t\t\r\n\t\t\t\t\t\t\t\r\n\t\t\t\t\t\t\t\tWeek 9 | \r\n\t\t\t\t\t\t\t\r\n\t\t\t\t\t\t\t\tLecture | \r\n\t\t\t\t\t\t\t\r\n\t\t\t\t\t\t\t\tLecture | \r\n\t\t\t\t\t\t\t\r\n\t\t\t\t\t\t\t\tTutorial | \r\n\t\t\t\t\t\t \r\n\t\t\t\t\t\t\r\n\t\t\t\t\t\t\t\r\n\t\t\t\t\t\t\t\tWeek 10 | \r\n\t\t\t\t\t\t\t\r\n\t\t\t\t\t\t\t\tLecture | \r\n\t\t\t\t\t\t\t\r\n\t\t\t\t\t\t\t\tLecture | \r\n\t\t\t\t\t\t\t\r\n\t\t\t\t\t\t\t\tTutorial | \r\n\t\t\t\t\t\t \r\n\t\t\t\t\t\t\r\n\t\t\t\t\t\t\t\r\n\t\t\t\t\t\t\t\tWeek 11 | \r\n\t\t\t\t\t\t\t\r\n\t\t\t\t\t\t\t\tLecture | \r\n\t\t\t\t\t\t\t\r\n\t\t\t\t\t\t\t\tTest 2 | \r\n\t\t\t\t\t\t\t\r\n\t\t\t\t\t\t\t\tTutorial | \r\n\t\t\t\t\t\t \r\n\t\t\t\t\t\t\r\n\t\t\t\t\t\t\t\r\n\t\t\t\t\t\t\t\tWeek 12 | \r\n\t\t\t\t\t\t\t\r\n\t\t\t\t\t\t\t\tLecture | \r\n\t\t\t\t\t\t\t\r\n\t\t\t\t\t\t\t\tLecture | \r\n\t\t\t\t\t\t\t\r\n\t\t\t\t\t\t\t\tStudent Presentation | \r\n\t\t\t\t\t\t \r\n\t\t\t\t\t\t\r\n\t\t\t\t\t\t\t\r\n\t\t\t\t\t\t\t\tWeek 13 | \r\n\t\t\t\t\t\t\t\r\n\t\t\t\t\t\t\t\tLecture | \r\n\t\t\t\t\t\t\t\r\n\t\t\t\t\t\t\t\tStudent Presentation | \r\n\t\t\t\t\t\t\t\r\n\t\t\t\t\t\t\t\tTutorial | \r\n\t\t\t\t\t\t \r\n\t\t\t\t\t\r\n\t\t\t\t \r\n\t\t\t | \r\n\t\t
\r\n\t\r\n
\r\n
\r\n
\r\n","Order":4},{"ID":"6e5f053b-8835-4692-be49-41f07234cfff","Title":"Syllabus","Description":"Introduction (3 hours)
\r\nDefinition and history of enzyme and enzyme catalysis, prejudices and advantages of enzyme catalysis, chemo-, regio-, and enantioselectivity, biotransformation with isolated enzyme and whole cells, industrial application, enzyme assay; enzyme activity, whole cell activity, space-time-yield.
\r\n
\r\nEnzyme classes and reactions (3 hours)
\r\nEnzyme classification (EC numbers), oxidoreductase, co-factor, transferase, hydrolase, lyase, isomerase, examples of enzymatic reactions.
\r\n
\r\nEnzyme selection (3 hours)
\r\nTarget reaction definition and selection, source of enzymes, enzyme or microorganism screening strategies, microtiter plate, high-throughput screening, detection methods, enantioselectivity assays.
\r\n
\r\nEnzyme purification and characterization (3 hours)
\r\nEnzyme purification techniques and general principles: SEC, IEC, HIC, IMAC, affinity matrices, affinity tags, and fusion proteins, examples. Enzyme characterization methods: molecular mass, IEP, multimers, amino terminal sequence, structure determination.
\r\n
\r\nEnzyme biochemistry and enantioselectivity (3 hours)
\r\nMichaelis-Menten kinetics, kinetic constants Kcat, Km, inhibition, enzyme selectivity, enantioselectivity, kinetic resolution vs dynamic resolution.
\r\n
\r\nEnzyme engineering (5 hours)
\r\nPhysicochemical classification of amino acids, replacement strategy, Dayhoff matrix, in vivo and in vitro mutagenesis (random, semi random), site directed mutagenesis, sequence comparisons and predictions, directed evolution, DNA shuffling, examples.
\r\n
\r\nBiotransformation with whole cells (3 hours)
\r\nCell growth, whole cells biotransformation, recombinant microorganisms, Multi-enzyme reactions, secondary metabolites, fermentation.
\r\n
\r\nBiotransformation with enzymes (3 hours)
\r\nCofactor regeneration systems (NADPH, NADH, ATP), coupled substrate approach, coupled enzyme approach, permeabilized cells, enzyme stabilization, immobilization technology, membrane reactors.
\r\n
\r\nBiotransformation in organic solvent (4 hours)
\r\nEnzymes and solvents: solubility of enzymes, substrates and products, water activity. organic solvents: ‘Klibanov’ rules, reaction reversal, ‘memory’ effect. Ion-paired enzyme. Enzyme catalysis in ionic liquid.
\r\n
\r\nProduct recovery (3 hours)
\r\nProduct recovery strategies, intracellular and extra-cellular compounds, disruption of cells, separation of cells, primary isolation (solvent extraction, sorption, adsorption, precipitation, membrane filtration, reverse osmosis, pervaporation, perstraction), further purification.
\r\n
\r\nProcess economics (3 hours)
\r\nProcess design, flow sheets, calculations, stringent and relaxed parameters, process evaluation, quantitation, economics of a process, research choices based on process cost considerations.
\r\n
\r\nBiotransformation in industry (3 hours)
\r\nCase studies based on operational industrial processes. ","Order":6},{"ID":"8e5f053b-8835-4692-be49-41f07234cfff","Title":"Assessment","Description":"CA: 50% [Test 1, 15%; Test 2, 15%; Term paper, 10%; Presentation (a selected journal paper), 10%]
\r\nFinal exam: 50%
\r\n","Order":8},{"ID":"6323e41d-80fc-40fc-a4b0-6a761224b3ff","Title":"Workload","Description":"3-1-0-2-4
\r\n
\r\nWorkload Components : A-B-C-D-E
\r\nA: no. of lecture hours per week
\r\nB: no. of tutorial hours per week
\r\nC: no. of lab hours per week
\r\nD: no. of hours for projects, assignments, fieldwork etc per week
\r\nE: no. of hours for preparatory work by a student per week","Order":9}],"ReadingFormatted":[{"ID":"27407ee2-fc14-47ee-894c-5004078a8ae7","Title":"Biocatalysts and Enzyme Technology","Author":"Buchholz, K.; Kasche, V.; Bornscheuer, U. T.","Edition":"","PubYear":"2005","ISBN":"3527304975","Publisher":"Wiley-VCH Verlag","BookType":"References","AdditionalInfo":"","CompWebsite":"","Order":1},{"ID":"64f2dde9-1941-4f77-9367-5caf011a696b","Title":"Biocatalysis","Author":"Bommarius, A. S.; Riebel B. R.","Edition":"","PubYear":"2004","ISBN":"9783527303441","Publisher":"Wiley-VCH Verlag","BookType":"References","AdditionalInfo":"","CompWebsite":"","Order":2},{"ID":"e7438abf-bfc0-4267-81b9-26182e5a6fb1","Title":"Applied Biocatalysis","Author":"Straathof, A. J. J.; Adlerkreuz, P.","Edition":"","PubYear":"2000","ISBN":"9058230244","Publisher":"Harwood Academic Publishers","BookType":"References","AdditionalInfo":"","CompWebsite":"","Order":3}],"ReadingUnformatted":[]}],"Lecturers":["Li Zhi"],"LecturePeriods":["Monday Morning","Wednesday Morning"],"TutorialPeriods":["Wednesday Morning"]}]}